Let’s grow a moth orchid(phalaenopsis)
(From non-symbiotic seeding to flowering)
Osaka Prefectural Engei Horticulture High School
2-5-1,Hachioji,Ikeda,Osaka 563-0037 Japan
Flower Factory course, Biotechnology club
E mail |
a_adachi@engei.osaka-c.ed.jp |
Phalaenopsis is an orchid family. It takes four years to produce flowers after seeding. We will show how it grows for the four years. |
A procedure for cultivation of phalaenopsis(Our way) |
1.Crossing |
Put pollinium on the back of the colum. When it works,a pod grows. Age the pod for threemonths |
2.Seeding (3 months after crossing) |
Put seeds from the pod on a hyponex substrate containing apple juice. |
3.First Subculture(3 months after seeding) |
Make the sobculture of the plant for the first time. |
4.Second Subculture(9 months after the first subculture) |
Mack the subculture of the plant for the second time. Use the same sobstrate as the first subculture. |
5.Planting out of the flask(9 months after the second subculture) |
Put the nursery plant out of the flask.After winding sphagnum moss around the plants,plant the 5-10 nursery plants together on a pan(φ12cm). |
6.First Replanting(1 year after planting out of the flask) |
Replant each nursery plant into a pot(φ7.5cm). |
7.Seocnd Replanting and flowering(1 year after the first replanting) |
Replant the plant into a bigger pot(φ12cm).The plant will come into bloom early in spring when the temperature rises. |
The above is the usual culture procedure.However,in our schoolwe have discovered a way to shorten the period from crossing to subculture(2months instead of 2 years).By using phytohormone,we grow high buds on the flower stalk and plant them. |
The Procedure for the formation of high buds of phalaenopsis |
1.Adjustement of flower stalk |
Cut the flower stalk 2cm long up and down from the bud. |
2.The first cultivation |
Plant the flower stalk into MSsubstrate added benzyl adenine andnaphthaleeaecetic acid.Benzyladenineand naphthaleeacetic acid isconcentrated to the level indicated inthe left figure. |
3.The second cultivation(1 month after the first cultivation) |
Plant the flower stalk for the secondtime into MS substrate addedbenzyladenine and naphthaleeaceticacid.Benzyladenine andnaphthaleeacetic acid is concentratedto the level indicated in the left figure. |
4.Planting out of the flask(1 month after the second cultivation) |
Put the nursery plant out of the flaskand replant into a pot(φ7.5cm)filledwith sphagnum moss.The laterprocess is the same as the usualculture procedure. |
If you have any questions or comments,email at | a_adachi@engei.osaka-c.ed.jp |