|
|
|
|
|
Product |
Reactivity |
Souce |
Type |
Dilution |
Pretreatment |
|
Cytokeratin |
cytokeratin No10,14,16,19/1-2,5 |
ICN |
mouse |
1：500 |
Proteinase K |
|
Cytokeratin |
cytokeratin No 8.18.19 . |
Becton |
mouse |
prediluted |
Proteinase K |
|
Cytokeratin |
55-57K |
Immunotech |
mouse |
prediluted |
Proteinase K |
|
Cytokeratin 19 |
cytokeratin No19(40KD) |
Oncogene |
mouse |
1：100 |
micro wave pH6.0 |
|
Cytokeratin 20 |
micro wave pH6.0 |
||||
|
Cytokeratin 5/6 |
micro wave pH6.0 |
||||
|
Cytokeratin 7 |
micro wave pH6.0 |
||||
|
Cytokeratin 34βE12 |
cytokeratin No 1,5,10,14 |
DAKO |
mouse |
prediluted |
micro wave pH6.0 |
|
Cytokeratin DE-K13 |
cytokeratin No 10,13 squamous cells |
DAKO |
mouse |
prediluted |
|
|
EMA |
epithelial cells,occasionarl plasma cells will be stained |
DAKO |
mouse |
1：200 |
|
|
E-Cadherin |
|||||
|
Collagen type3 |
basement membranes |
DAKO |
|||
|
LCA |
lymphocyte,ML and leukaemia of B and T types,Hairy cells |
DAKO |
mouse |
1：200 |
|
|
L-26(CD20/cy) |
B cells,PanB |
DAKO |
mouse |
1：200 |
|
|
CD79(JCB117) |
preB cells,PanB |
DAKO |
mouse |
1：350 |
water bath pH9.0 |
|
LN-3 |
HLA Class II (DR) |
Techniclone |
mouse |
prediluted |
|
|
UCHL-1(CD45R) |
helper T cell(40%) |
DAKO |
mouse |
1：400 |
|
|
MT1(CD43) |
reacts with all T cells |
BioScience |
mouse |
1：200 |
|
|
IgA |
IgA of immunoglobulin |
Zymed |
mouse |
prediluted |
Proteinase K |
|
IgM |
IgM of immunoglobulin |
Zymed |
mouse |
prediluted |
Proteinase K |
|
IgG |
IgG of immunoglobulin |
Zymed |
mouse |
prediluted |
Proteinase K |
|
kappa |
Kappa Light Chain |
Zymed |
mouse |
prediluted |
Proteinase K |
|
Lambda |
Lambda Light Chain |
Zymed |
mouse |
prediluted |
Proteinase K |
|
Cyclin D1 |
Mantle cell lymphomas,30% of breast cacers Nuclear staining of tumor cells |
DAKO |
mouse |
1：100 |
water bath pH9.0 |
|
bcl-2 |
Follicular lymphomas |
ニチレイ |
mouse |
prediluted |
water bath pH9.0 |
|
CD3 |
mature T cell,ATL,T-CLL,PanT |
DAKO |
rabbit |
1：300 |
water bath pH9.0 |
|
CD4 |
Herper/inducer Tcells |
ニチレイ |
mouse |
prediluted |
water bath pH9.0 |
|
CD5 |
thymocytes,peripheral blood lymphocytes |
Novo |
mouse |
1：50 |
water bath pH9.0 |
|
CD8 |
Suppressor/cytotoxic T cells |
Novo |
mouse |
1：300 |
water bath pH9.0 |
|
CD30,Ki-1 |
activated lymphoid cells,Hodgkinand Reed Sternberg cells |
DAKO |
mouse |
1：1000 |
water bath pH9.0 |
|
CD10 |
Common Acute Lymphoblastic Leukaemia antigen(CALLA),CML ,breast myoepithelial cell,bile canaliculi,fibroblasts |
Novo |
mouse |
1：100 |
water bath pH9.0 |
|
ALK1 |
p80,ALK1 protein. |
DAKO |
mouse |
1：50 |
water bath pH9.0 |
|
Ki-67 |
proliferrating cells,late G1,S,M and G2 phases |
Immunotech |
mouse |
1：150 |
water bath pH9.0 |
|
CD56(NCAM) |
Neurons,Astrocytes,Schwann cells,NK cells |
Novo |
mouse |
1:50 |
micro wave pH6.0 |
|
CD57 (Leu7) |
Becton |
mouse |
prediluted |
||
|
BM-1 |
granulocytes,myelocytes |
ニチレイ |
mouse |
predilutid |
|
|
p53 protein |
wild type and mutant forms of p53 protein |
NOVO |
mouse |
1：200 |
water bath pH9.0 |
|
KP1,CD68 |
macrophage,monocyte,and myeloid origin |
DAKO |
mouse |
1：200 |
Proteinase K |
|
Vimentin |
cells of mesenchymal origin. Lymphoid cells,endothelial cells,fibroblasts |
DAKO |
mouse |
1：40 |
micro wave pH6.0 |
|
Desmin |
rhabdomyosarcoma and leiomyosarcoma marker |
DAKO |
mouse |
1：50 |
micro wave pH6.0 |
|
c-kit(CD117) |
c-kit oncoprotein,GIST |
DAKO |
rabbit |
1：50 |
|
|
Musle Actin, |
Alpha-Sarcomeric Muscle Actin |
DAKO |
mouse |
1：50 |
|
|
Myf-3 |
Rhabdomyosarcoma Marker |
Novo |
mouse |
1：50 |
micro wave pH6.0 |
|
Alpha-Smooth |
striated muscle cells |
DAKO |
mouse |
1：100 |
|
|
Myoglobin |
rhabdomyosarcoma marker |
Novo |
rabbit |
1：500 |
micro wave pH6.0 |
|
CD34 |
endotherial cell marker |
Novo |
mouse |
1：35 |
micro wave pH6.0 |
|
CD99(MIC2) |
Ewing's Sarcoma marker,pPNET |
DAKO |
mouse |
1：100 |
|
|
Amyloid P Component |
Amyloidsis,amyloid p Component,C1t complement,α1-glycoprotein |
DAKO |
rabbit |
1：200 |
|
|
Amyloid A component |
AA type amyloidosis |
DAKO |
mouse |
1：100 |
|
|
M-GGMC-1(HIK1083) |
Adenoma malignum of uterine cervix gastric mucin type3 |
Merk |
mouse |
1：50 |
micro wave pH6.0 |
|
HGM-45M1 |
Human Gastrin Mucin |
mouse |
1：50 |
||
|
MUC2 |
The intestinal apomucin. Muc2-Glycoprotein |
Novo |
mouse |
1：100 |
|
|
SIMA |
Small intestinal mucinous antigen,goblet cells |
Novo |
mouse |
1：100 |
|
|
CA-125 |
Ovarian cancer marker |
TBF |
mouse |
prediluted |
|
|
CA-153 |
Breast cancer marker |
TBF |
mouse |
prediluted |
|
|
CEA |
cancer cells marker |
IBL |
rabbit |
1：50 |
|
|
AFP |
hepatoma and yolk sac carcinoma |
ニチレイ |
mouse |
prediluted |
|
|
CA19-9 |
(Sialyl Lewis) pancreas cancer |
TBF |
mouse |
prediluted |
|
|
NSE |
Oat cell carcinoma,Neuroendocrine tumors |
Novo |
mouse |
1：50 |
micro wave pH6.0 |
|
Neuroblastoma Marker |
Neuroblastoma(90%),Ewing's sarcoma(50%) |
Novo |
mouse |
1：100 |
|
|
Neurofilament |
68KD,160KD,200KD |
Immunotech |
mouse |
prediluted |
micro wave pH6.0 |
|
Chromogranin |
Neuroendocrine tumors |
Milab |
rabbit |
1：500 |
|
|
Synaptophsin |
Neuroendocrine tumor,neuroblastomas |
DAKO |
rabbit |
1：50 |
micro wave pH6.0 |
|
S-100 |
glial cells,ependymal cells,schwann cells,melanocytes,langerhans cells |
DAKO |
rabbit |
1：400 |
|
|
Hepatocytes |
Hepatocytes,Hepatocellular carcinoma |
DAKO |
mouse |
1：50 |
micro wave pH6.0 |
|
HCG |
Human Chorionic Gonandotropin,placental trophoblasts |
DAKO |
rabbit |
prediluted |
|
|
Calcitonin |
Medullary carcinoma |
Milab |
rabbit |
１：500 |
|
|
GFAP |
gilial fibrillary acidic protein,astrocytomas and ependymomas |
DAKO |
rabbit |
prediluted |
|
|
Gastrin |
Gastrin-secreting tumors |
DAKO |
rabbit |
prediluted |
|
|
Gulcagon |
Gulcagonoma,Gulcagon-secreting tumors |
Milab |
rabbit |
1：500 |
|
|
Insulin |
Insulinomas,Insulin-secreting tumors |
ニチレイ |
mouse |
Prediluted |
|
|
Pancreatic polypeptide |
pancreatic islets |
DAKO |
rabbit |
1：300 |
|
|
Somatostatin |
Somatostatinoma,Somatostatin-secreting tumors |
DAKO |
rabbit |
predilutedc |
|
|
Serotonin |
5-HT producing cells,Neuroendocrine cells |
Sera-Lab |
rat |
1：600 |
|
|
PSA |
(Prostatic Specific Antigen) prostatic carcinoma |
DAKO |
rabbit |
prediluted |
|
|
PSAP |
(Prostatic Acid Phosphatase),Prostatic carcinoma,carcinoid tumor |
DAKO |
rabbit |
prediluted |
|
|
ACTH |
adrenocorticotrophic hormone,pituitary adenoma |
DAKO |
rabbit |
prediluted |
|
|
HMB-45 |
melanoma cells |
DAKO |
mouse |
1：50 |
micro wave pH6.0 |
|
pS2 Protein |
estrogen responsive breast cancer cell lines. gastric mucosa,small intestinal mucosa |
Novo |
rabbit |
１：500 |
|
|
PgR |
Progesteron Receptor |
Immunotech |
mouse |
prediluted |
pH6.0 95℃,40min |
|
ER |
Estrogen Receptor |
Immunotech |
mouse |
prediluted |
pH6.0 95℃,40min |
|
Hercept Test |
HER2/neu |
DAKO kit |
rabbit |
Prediluted |
water bath pH6.0 95℃,40min |
|
CMV(CCH２＋DDG9) |
Cytomegalovirus |
DAKO |
mouse |
1：50 |
(proteinase K) |
|
EBV(EBNA2,PE2) |
Epstein-Barr Virous |
DAKO |
mouse |
1：50 |
micro wave pH6.0 |